The film revolves around two American tourists, Lindsay (Ashley C. Williams) and Jenny (Ashlynn Yennie), who find themselves stranded in rural Germany. They stumble upon the eerie and isolated home of Dr. Heiter (Dieter Laser), a former surgeon with a twisted obsession: to create a human centipede by surgically connecting the mouths of three individuals, thereby creating a grotesque, interconnected digestive system.