The Preludio Sinfonico is structured as a single, cohesive piece that explores various themes and moods. It features a rich orchestration, characteristic of Puccini's skill in handling large musical ensembles. The work begins with a somber introduction, gradually evolving into a more dynamic and emotionally charged sequence of themes. These themes are developed throughout the piece, leading to a dramatic climax before concluding with a serene and uplifting coda.